Is there any way to design allele specific PCR primers for a single nucleotide "deletion"?

Hi, I am currently doing a research on Inborn errors of metabolism in Sri Lanka and for that, I need to develop a method to identify few SNPs and a single point deletion using allele specific PCR...

27 August 2020 2,411 4 View

How to evaluate the accuracy of unupervised machine learning algorithms ?

What are the ways that we can use to analyze the accuracy of unsupervised anomaly detection algorithms like HBOS or Isolation Forest ?

24 February 2020 5,393 3 View

How to Encode an IBAN Number ?

My dataset has an IBAN number column with the following format. IBAN Number Example :- "GB38MIDL400530543464" What is the best way to encode the IBAN number column before training the dataset?

02 February 2020 2,793 3 View

Do any one knows about Non linear modeling techniques for behavioral modelling?

I'm exploring non linear modelling technique for demand forecasting in transportation sector where alternative transport mode implement. As a start it is good to have some ideas from you Thank you

18 October 2018 7,019 1 View

Possible experiment to check protein solubility in cell culture model?

I am looking to analyze how the solubility of aggregation-prone proteins (like Tau, A-beta or a-synuclein) behave under different protein disaggregases and chaperons. Something along the line of...

13 September 2018 8,263 1 View

Suggestions for the questionnaire ?

Currently I am doing a research on Customer Retention Hotel Service Attributes in Sri Lanka: Effect of Customer's Nationality - Case of Cinnamon Grand Hotel. with the support of literature i...

11 December 2017 5,823 7 View

How to enhance your in vivo photo-cross-linking experiments in E.coli?

13 October 2016 6,157 5 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View