Difference between Bray-Curtis dissimilarity and Unifrac phylogenetic distance for visualizing microbial community differences?

Can someone explain this figure based on cluster analysis of different soil samples based on OTU abundance for each soil.what is the impact of genetic distance between different treatment samples?

04 May 2018 2,905 2 View

Best staining methods to ensure the effective AMF inoculation?

I am working on Arbuscular Mycorrhizal Fungi (AMF) with emphasizing on Cucumber productivity in the presence or co-inoculation with organic substrate, Could someone guide how to check the...

04 May 2018 8,787 9 View

High throughout microbial sequencing approaches; pyrosequencing, illumina can ensure the effectiveness of collected soil samples ?

By using these methods, DNA extraction, PCR amplification and bioinformatics assay are core steps to process the sequence analysis. After process the data, we have a abundant information regarding...

03 April 2018 4,480 10 View

What is the significance value of particular microbial diversity?

To explore the soil microbial diversity, usually we analyse the alpha and beta-diversity of microbes. what is the significance of both indicators, what these indices explicit the true microbial...

03 April 2018 1,518 0 View

The role of plant residues in pH change of acidic/alkaline or neutral soils ?

Plant residue having 7.25 pH added in soil, what will be the impact on soil acidity or alkalinity, Residue alkaline in nature could increase or decrease the soil status it term of pH and what...

11 December 2017 8,719 5 View

What will the impact of high TOC on soil quality? Does it effect CN ratio?

if plant residues have high TOC (412g/kg), after incorporating into soil, what will effect on soil quality? and does high TOC effect CN ratio? please explain it.

10 November 2017 5,648 8 View

If the plant residue amended in soil with high CN, How to control C:N ratio after applied in soil?

Pot soil amended with plant residue for short duration cucumber growth. if plant residue has high CN ratio (OC: 371g/kg, N:0.97 g/kg). So in that case how to control/balance or lower down the...

10 November 2017 1,411 6 View

Is there any approach to measure biochar as a soil quality indicator? Is it possible to measure the biochar contents as a parameter not as treatment?

In generally, we apply biochar as a treatment for soil amendment. Is it possible to take it as parameter (as soil quality indicator) not as treatment. need valuable guideline, any protocol?

10 November 2017 861 12 View

How much soil is is present at 30cm depth in one hectare?

05 June 2016 4,525 6 View

Can someone explain that determination of microbial population and diversity as influenced by applied plant residues, How much be affected?

I have applied plant residues (Raw Garlic stalk) with 3 different concentrations in cucumber continuous potted soil. i want to check its efficacy towards microbs addition or not? i want to...

01 January 1970 795 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

Where can I find Plasmopara viticola?

Hello everyone, For my research I want to infect Vitis Vinifera leaves with downy mildew-causing fungus Plasmopara viticola. Does anyone know where I could find or purchase this fungi? I am...

04 February 2021 5,720 3 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View