How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

I want to convert Methane gas to liquid Methanol using photocatalysis. Can you please suggest me a good paper which I can study ?

There are a number of publications regarding this, but none of them are clear and show the mechanism of gaseous Methane conversion to liquid Methanol.

06 January 2021 364 3 View

Can wavelet packet sub-bands be stacked together to form an image?

I have a 1D signal and I have done wavelet packet decomposition on it which is giving several sub-bands. Can I stack these sub-bands (one below other) to form a 2D matrix and hence an image...

27 November 2020 1,269 5 View

How can I maintain a surface at isothermal condition, say at 300 deg Celsius.?

I have 6 heaters each of power 0.5 kW and mounted below a curved mild steel sheet of thickness 4 mm. The heaters and the below section is covered and insulated. I want the top surface to be...

05 November 2020 199 5 View

what are the possible reasons for Crop Water Stress Index values higher than 1 under moisture stress condition?

I have determined CWSI using the theoretical approach of Jackson et al., (1982) based on canopy temperature measurement in chickpea. at the final stages of crop growth some of my values of CWSI...

28 October 2020 9,992 3 View

Do always a Good paper depends on How much Citations it Received. I have seen many Good Research Papers with zero or less Than 10 Citations?

Its a Research Paper related Question

22 October 2020 6,179 4 View

Help in cloning, should I create 5' phosphate group on digested vector?

Hello all, I am trying to introduce an annealed DNA fragment into the double digested vector. The ssDNAs were ordered without a phosphate group modification. The vector is digested with XhoI and...

20 October 2020 8,357 5 View

Can anyone explain the calculation process of integrated biomarker response (IBR) used in chronic toxicity study with an arbitrary dataset?

Video tutorial on IBR calculation or stepwise procedure..

05 October 2020 9,279 3 View

Could someone provide the permeability value for 2% Chromium doped BiFeO3?

Any close value will also work.

03 October 2020 8,067 4 View

Purification of annealed oligonucleotides after annealing procedure?

Hello all, I will do oligonucleotides annealing for the first time which will be later used for cloning without any more modification. Can any of you kindly suggest if I should purify the annealed...

16 September 2020 3,881 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View