What is the optimum working concentration of phytohormones like IAA, NAA, Kinetin and BAP in MS media for Arabidopsis to do phenotypic characterization?
If you want to characterise different genotypes, you should avoid plant growth regulators. Half-strength basal MS with 3% sucrose is sufficient to grow them in tissue culture.
I agree with Ranjith. We routinely use 1/2 MS with 0.8% agarose (or agar) and sometimes without sucrose. When we use sucrose, we use it at 1%, but 3% is good too. We don't use plant growth regulators unless we are trying to induce callus or regenerate explants.
Thanks for the response Dr. Ranjith and Dr. Oppenheimer. But we were trying to study the influence of various growth regulators in the light response of plants. This doesn't involve tissue culture and the basic interest is not callus induction or regeneration.
Dear Yadukrishnan,
If you are planning to grow your plants in a hydroponic system, MS is too rich. You should use Hoagland's or Knop's solution. The ratios and concentrations of plant growth regulators have to be designed after a thorough literature review. Suggested ratios control, 0:0.5, 0:1, 0:2, 0.5:0, 0.5:0.5, 0.5:1, 0.5:2 and so on.
Good luck and happy new year!
Ranjith
I think it depends on the type of plant growth regulators used in media.
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