What is the optimum culture condition of the MCF10A ( for ATCC CRL 10317) breast cell line ? Is there any option to prepare medium for MCF10A 'without' cholera toxin ? There is serious import/export problems of cholera toxin to have.
I remember to have cultivated them in normal DMEM medium with 10 % FBS and they were fine.
I have already contacted the ATCC for my MCF 10A cells (ATCC® CRL-10317™). They strongly recommend the inclusion of cholera toxin when growing up the cell line because of that cholera toxin works as a growth promoting supplement. They have said there is a chance that the cells will grow fine without the cholera toxin supplement, but ATCC has never tried to grow these cells in medium lacking the cholera toxin.
Additionally, they send me a reference without cholera toxin. According to the original reference for this cell line, without the cholera toxin, the cells will grow in dome formations. A link to the reference can be found below:
http://cancerres.aacrjournals.org/content/canres/50/18/6075.full.pdf
There are no more information on ‘without cholera toxin’. Tarek Adnan Ahmad
Christian GabrielThank you for your replies.
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