What are the effective tech-collaborative-tasks for developing reading skill?

Collaboration is essential for ensuring learning for all learners in one classroom. Blackboard collaboration tools like blogs, wikis can be considered the best platform for increasing interaction...

17 February 2020 2,991 3 View

Why my PCR band is so weak?

I do PCR reaction for my gene (it's 413bp). my pcr bands are so weak. I examined different template concentration (template is FMDV cDNA, 4900 nt),increase Mgcl2 concentration, and reduce primeres...

04 December 2018 4,772 15 View

Does ABAQUS software give me sound absorption coefficient of the materials directly without adding any any code itself?

13 February 2016 8,286 10 View

In all optical autocorrelation for femto-second pulse measurement: Why is a nonlinear SHG crystal situated in front of the detector?

01 January 2016 7,340 3 View

How do I evaluate the QRS peaks detector?

14 September 2015 2,574 15 View

What are the advantages and disadvantages of Stable versus unstable resonator?

05 March 2015 8,961 4 View

Is there any relation between coherence and polarization as two distinct properties of light?

To my knowledge, there is no solid correlation between temporal or spatial coherence and the polarization of light (linear, circular or elliptical) because the coherence arises from the nature of...

04 January 2015 3,902 12 View

Does anyone know how to make Fluorapatite with Phosphoric Acid?

I am Interested in making Fluorapatite.

24 August 2014 3,948 8 View

Does the primary Ab and secondary Ab from different companies matter?

I am using cell signalling primary antibody and secondary antibody from sigma and substrate is NBT/BCIP. primary Ab dilution 1:1000 (3hrs rocking) sec Ab 1:10000 (45min rocking) primary ab...

14 March 2014 7,713 5 View

How do you add output styles in Endnote web?

I want to add the XXX journal output style in endnote web. In my endnote web only 21 output style are available, e.g. numbered, Ris, science, etc.

09 March 2014 1,536 11 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

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02 March 2021 3,060 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View