What are the effective tech-collaborative-tasks for developing reading skill?

Collaboration is essential for ensuring learning for all learners in one classroom. Blackboard collaboration tools like blogs, wikis can be considered the best platform for increasing interaction...

17 February 2020 2,991 3 View

Why my PCR band is so weak?

I do PCR reaction for my gene (it's 413bp). my pcr bands are so weak. I examined different template concentration (template is FMDV cDNA, 4900 nt),increase Mgcl2 concentration, and reduce primeres...

04 December 2018 4,772 15 View

Does ABAQUS software give me sound absorption coefficient of the materials directly without adding any any code itself?

13 February 2016 8,286 10 View

In all optical autocorrelation for femto-second pulse measurement: Why is a nonlinear SHG crystal situated in front of the detector?

01 January 2016 7,340 3 View

What are the advantages and disadvantages of Stable versus unstable resonator?

05 March 2015 8,961 4 View

Is there any relation between coherence and polarization as two distinct properties of light?

To my knowledge, there is no solid correlation between temporal or spatial coherence and the polarization of light (linear, circular or elliptical) because the coherence arises from the nature of...

04 January 2015 3,902 12 View

Does anyone know how to make Fluorapatite with Phosphoric Acid?

I am Interested in making Fluorapatite.

24 August 2014 3,948 8 View

Does the primary Ab and secondary Ab from different companies matter?

I am using cell signalling primary antibody and secondary antibody from sigma and substrate is NBT/BCIP. primary Ab dilution 1:1000 (3hrs rocking) sec Ab 1:10000 (45min rocking) primary ab...

14 March 2014 7,713 5 View

How do you add output styles in Endnote web?

I want to add the XXX journal output style in endnote web. In my endnote web only 21 output style are available, e.g. numbered, Ris, science, etc.

09 March 2014 1,536 11 View

What might be the reason for ghost bands in western blot?

What might be the reason for ghost bands in western blot? 1. I have used 0.5% skim milk overnight blocking 2. Primary antibody dilution 1:1000 3hrs rocking (3xwash PBST/ PBS) RT 3.Secondary...

06 February 2014 4,389 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

MVN, how to conciliate contradictory results from Multivariate normality and Multivariate outliers test?

Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...

18 February 2021 190 3 View

Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Can we have constructs of Cronbach alpha value with more than 0.9 in social science research? What is recommended in this problem?

My study consists of a conceptual framework with seven constructs measured in seven point Likert scale. According to pilot study, only three constructs have Cronbach alpha value more than 0.9...

12 February 2021 9,368 2 View

Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...

08 February 2021 9,671 3 View

Does Gaussian 09W software take too long to give a result if we have a large chemical structure ?

DFT - gaussviev - gaussian09W - HOMO - LUMO

08 February 2021 6,012 5 View

How can I select the best plasmid after sequencing?

I have transformed a drought-related gene of 1.7kb into pGEMt vector (E.coli DH5 alpha strain). After sequencing the plasmids (3 replicates) 4 changed amino acids are visible at different...

06 February 2021 336 3 View