Asiya Parvin

10 Questions 13 Answers 0 Followers

Questions related from Asiya Parvin

Does Akt band show a decreased band intensity if there is an increase in phosphorylation of Akt?

I have used Akt as control (equal amount of protein loading) for p Akt . The blot showed no change in Akt level on all four lanes but p Akt level got increased in treated lane and decreased in...

16 June 2015 6,648 12 View

Does the primary Ab and secondary Ab from different companies matter?

I am using cell signalling primary antibody and secondary antibody from sigma and substrate is NBT/BCIP. primary Ab dilution 1:1000 (3hrs rocking) sec Ab 1:10000 (45min rocking) primary ab...

15 March 2014 7,742 5 View

How do you add output styles in Endnote web?

I want to add the XXX journal output style in endnote web. In my endnote web only 21 output style are available, e.g. numbered, Ris, science, etc.

10 March 2014 1,586 11 View

What might be the reason for ghost bands in western blot?

What might be the reason for ghost bands in western blot? 1. I have used 0.5% skim milk overnight blocking 2. Primary antibody dilution 1:1000 3hrs rocking (3xwash PBST/ PBS) RT 3.Secondary...

07 February 2014 4,423 5 View

Why are bands are not visible in PVDF membrane after being stained with ponceau?

I have transferred gel in PVDF membrane and stained with ponceau but I couldn't see any bands on the membrane, instead, the whole PVDF membrane is turned into pink color and the color is not going...

30 December 2013 7,392 2 View

What is the optimized protocol for loading Fluo-4 AM (calcium indicator) in myocytes?

I am not experienced with Calcium imaging. I would like to load Fluo-4 AM in myocytes and check the Ca transit and spark. I read some articles most of them have used patch clamp method. Is it...

20 September 2013 5,292 2 View

How to do pI3 kinase assay by using PI3 Kinase Activity/Inhibitor Assay Kit (millipore)?

I want to do PI3 Kinase Activity assay by using millipore PI3 Kinase Activity/Inhibitor ELISA kit in that protocol they didn't mention the details about the sample volume in the the...

06 July 2012 6,565 1 View

Can I use secondary antimouse IgM for primary Phospho-Akt (Ser473) (587F11) Mouse mAb #4051?

Can i use secondary antimouse IgM for primary Phospho-Akt (Ser473) (587F11) Mouse mAb #405v1 for western blot?

06 July 2012 4,779 12 View

What is the step by step protocol for DCF assay?

I searched in many article i want to measure Ros during cocl2 treatment after 24 hr after drug pre treatment in adherent cells by using plate reader and also in con focal but i am confused...

25 June 2012 6,623 3 View

How to calculate 100 microMolar solution of COCl2. 6H2O?

M.Wt of COCl2.6H20 is 237.93 For stock sol 25mM 5.95mg dissloved in 1ml ddH20 if i take 4 microliter from the stock and make up into 1ml can i get 100microMolar solution? If i take 4 from the...

02 June 2012 8,582 7 View