Can anybody suggest the optimal concentration and time needed for MG132 treatment in HUVECs, infected with Adenovirus expressing a protein that gets ubiquitinated and the protein's biological Half-life is 70min?
Stop manmade dangers and team up to face climatic danger. The Palestine solidarity groups, there are many group in it, think they can make UK and USA to order Palestine a free state. Palestine...
01 April 2024 2,137 1 View
Seeking for a method
10 December 2023 915 0 View
I have already analysed optical properties of other compounds successfully. But for this one I’m getting this type of error as shown in the attached image. Can anyone assist me regarding the problem?
16 August 2023 5,594 0 View
The precursors are SnCl2.2H2O, PbCl2, and water as solvent.
11 August 2023 8,620 3 View
I did SiO2 dry etching using CHF3 (100 W RIE power, 10 mT pressure, 20 sccm CHF3 flow) at 20C. After the etch process, I noticed cracks/peeling off in multiple places of my sample (image...
18 July 2023 7,663 3 View
There it remains unspecified about, among those two legged beings, before snow ball earth of ice age era, whether there were human beings and their traces not yet discovered due to having no...
06 May 2023 7,076 3 View
I transfected 293T cells with a flag tagged protein (40kDa). After performing the westernblot and Flag antibody treatment I was supposed to see a band on 41 kDa. Instead,that I see clear band on...
05 March 2023 5,634 3 View
whenever potential is applied at higher scan rates the active material comes out of the current collector and I'm unable to perform further tests such as GCD and Impedance.
16 February 2023 8,023 0 View
why my calcined sample shows more area under the curve as compared to the uncalcined one.
16 February 2023 4,601 1 View
Sea level rising and flood prone zone Bangladesh. Storm risk and coral reefs on risk damaging. High tide of waves on the increase. How Bangladesh building international airport on the seaside,...
24 December 2022 850 2 View
We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....
17 June 2024 1,649 0 View
We have seen in the literature that Ang-1 can be considered as one of the markers. We need information about few more markers.
01 June 2024 9,650 1 View
I am working with primary HUVEC cultures from PromoCell. They proliferate well using ECGM media with 2% FBS from PromoCell (Ref. C-22010), and the cells look healthy and grow successfully. Then,...
14 May 2024 376 1 View
I have sequence of Mitochondrial proteins at which ubiquitination occurs. i want to find out whether its located on MTS or not.
19 March 2024 8,964 0 View
Hi everyone I have access to some HUVECs from LONZA. However, I am considering using an alternative media instead of Lonza EGM Endothelial Cell Growth Medium BulletKit. Has anyone ever used...
27 November 2023 6,131 4 View
Dear community, our group is working with HUVECs for the first time and we are lacking some experience... Does anyone know the protein yield of HUVECs? Do you have an estimation how many...
23 November 2023 557 1 View
I make a concentration of 50 ng/mL VEGF in endothelial cell growth medium. I add this medium to my microfluidic device to stimulate HUVECs for angiogenesis. I was wondering how long I can use...
15 October 2023 4,838 1 View
We are starting a culture of HUVECs. We are successful growing them in the medium provide by the company (Sigma-Aldrich C-12200 Human Umbilical Vein Endothelial Cells, HUVEC) . But the "ready to...
15 October 2023 5,698 4 View
Hello, I'm using the DynaPro NanoStar DLS system. I was wondering if anyone had previous experience in characterizing aggregates for adenovirus. I'm measuring samples as is, i.e undiluted. My...
26 September 2023 3,936 10 View
I have been culturing HUVEC and sometimes the cells look stressed and stop dividing. When this happens, they don't recover after I change the medium. What may be causing this problem? Expired...
23 September 2023 2,577 3 View