Fatema Yeasmin Tanni

4 Questions 2 Answers 0 Followers

Questions related from Fatema Yeasmin Tanni

Can anyone suggest me some Pan-protease inhibitors to prevent protein cleavage?

I am working with a protein. Every time I do a Western blot, I see cleavage of my protein. Even when I overexpress the same protein tagged with GFP, I see the cleavage. I tried Pan-caspase...

31 January 2025 4,424 4 View

What is the optimal concentration for MG132 treatment in HUVECs?

Can anybody suggest the optimal concentration and time needed for MG132 treatment in HUVECs, infected with Adenovirus expressing a protein that gets ubiquitinated and the protein's biological...

24 April 2024 4,419 0 View

Why do I get a low molecular weight band with flag tagged protein?

I transfected 293T cells with a flag tagged protein (40kDa). After performing the westernblot and Flag antibody treatment I was supposed to see a band on 41 kDa. Instead,that I see clear band on...

06 March 2023 5,652 3 View

For sequencing, is it necessary to extract Bacterial DNA by Genomic DNA extraction kit?

While doing PCR if someone finds perfect band for specific gene by using DNA template which was Extracted by boiling method, would the Extracted DNA template be good for sequencing? Or should the...

02 July 2022 7,457 3 View