I want to use a lipase from Candida rugosa as part of a histology procedure.
Would the lipase function in PBS pH 7.5 at 37C?
Would the lipase function in the presence of 8% Sodium dodecyl sulfate (SDS)?
There are a lof of papers that point to several optimal pHs ranging from 6.5-8.5, different buffers and temperatures. I would like to keep using a simple PBS, but I am curious if the lipase would function in the presence of the detergent SDS, or if they would have an additive effect and improve the fat breakdown.
I am afraid that SDS will denature your lipase. Why do not you use mild detergents instead of SDS? Non-ionic detergents, such as Triton X-100, is more preferable. You need to also use appropriate emulsifying agents in your reaction mixture. You can refer the materials and methods section of the paper for assay of the activity of lipases. here is the link. I hope it will help!
https://www.researchgate.net/publication/233579090_Increased_enantioselectivity_of_lipase_in_the_transesterification_of_DL--3-phenyllactic_acid_in_ionic_liquids
Article Increased enantioselectivity of lipase in the transesterific...
The SDS will denature the lipase/ protein. avoid using it in the reaction cocktail. Refer to the following article;
Kanwar SS, Kaushal RK, Jawed A and Chimni SS (2005). Evaluation of methods to inhibit lipase in colorimetric assay employing p-nitrophenyl palmitate. Indian J Biochemistry & Biophy 42: 233-237.
Candida rugosa works in PBS pH 7.5 at 37C. you can try some other detergents.
Thank you for the helpful answers. I'll omite the SDS at keep it simple with PBS pH 7.5 at 37C.
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