Hello!
I have a stock of BT474 human breast cancer cells, which we have received as a gift from another Institute, but I suspect that the cell line is impurified or something. It seems that the cells express two different phenotypes. I have attached a picture where the actin filaments are marked with Phalloidin.
Thank you!
hi
It does not matter . you can check your line by anti -Her2 antibody in FACS assay .
This line highly positive for this marker .
good luck
Thank you for your answer! At this moment I do not have the possibility to do this assay. I wanted to know the opinion of someone who has used this cell line (based on morphology).
Hi Roxana,
If you believe that your cell lines are impure, then you need to get them fingerprinted to ensure that they are BT474 cells. From working with the BT474 cells i remember them being quite a heterogeneous population and your images seem quite representative of the cell type. However before you start working with them, it is critical that you clarify that the stock you have are actually BT474 cells.
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