What is the Nessler method for determination of Urease activity ?

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Adam B Shapiro

Here is a protocol:

chrome-https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/253/079/urease.pdf

Nessler's reagent detects the ammonia produced by the action of urease on urea.

Paul Milham

Nessler's reagent contains the toxin, Hg, and has largely been superseded by reactions with phenols. The literature is readily found.