We know that there are many enzymes in the soil. Such as dehydrogenase, catalase, alkaline phosphatase, urease, b-glucosidase and other enzymes. How can they be detected in the laboratory?
Unfortunately , there is no short cut , except to follow the standard protocol, otherwise later , you will face problem in publishing the same in standard journals...
For soil enzyme activity you need minimum good grade reagents with proper washed glassware and spectrophotometer and also a freeze and finally a well mentained lab with skilled persons. So there is no cheap and simple way in that. To find out Enzyme activity in soil is one of the sofisticated and also interesting work.
IT DEPENDS UPON WHICH ENZYME YOU ARE TARGETTING THERE ARE FEW QUILITATIVE TESTSTO KNOW THE HYDROLITIC ENZYMES AND ALSO SOMER QUANTITATIVE SPECTROMETRIC ANALYTICAL TESTS
It depends on your research question. For example if you want to estimate redox activity then you measure dehydrogenase activity, If you are studying hydrocarbos contamination then measure lipase-esterase activity....and so on.
It mainly depends on the specific enzyme you want to test, and the method of measuring the activity of each enzyme is different.
For example, soil urease activity determination method: indophenol colorimetric method; soil protease activity determination method: copper salt colorimetric method; soil phosphatase determination method: benzene disodium phosphate method; soil sucrase determination method: 3,5-dinitrogen Salicylic acid colorimetric method; Determination method of polyphenol oxidase activity: pyrogallol colorimetric method.
In addition, here is an article about the activity overview. Maybe you are interested in reading it. https://www.cusabio.com/c-20943.html
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