Do you mean ammonium sulphate, rather than persulphate? Ammonium persulphate is used in polymerizing SDS-PAGE gels. Ammonium sulphate is used for non-denaturing protein precipitation.

Thank you Shapiro sir for your reply. Sorry, its Ammonium sulphate.

Good question,

No effect, the urease purifying precipitating agent is used ammonium sulfate and the solubilizing agent is used as Citrate to dissolve the precipitate. hence this the continuous process simultaneously dissolve precipitate by Citrate solubilizing agent. The enzyme precipitation using 60% saturation ratio of Ammonium sulfate gave the maximum activity of urease precipitation among other precipitation methods. Enzyme characterization appeared the optimum pH of urease activity was 8.0 and gave enzyme activity 93.8U/ml, while the optimum pH of urease stability is 6.0; the enzyme maintains its activity. The 50 % acetone is used for dissolve precipitation. Alcohol precipitation and Ammonium sulfate precipitation, these ways of urease precipitation gave enzyme activities 74.8; 43.6 and 82.2 U/ml respectively . Urease enzyme is precipitated using acetone 50% and the clear suspension of precipitated enzyme is collected and determined it’s enzyme activity which reached to 74.8U/ml in order to compare with other precipitation method.

Thank you very much sir for great reply.