What is the name of the research group in Paris, France, with experience in endophyte isolation from plants?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How do you feel about social exclusion in transport within South Africa?

Research being conducted by Stellenbosch University would like your opinion about the effects of social exclusion on emerging urban mobility options and traditional public transport services. By...

21 July 2024 4,032 0 View

Calculating the injection volume for a mouse?

I will be administering 5-fluorouracil at a dosage of 50 mg/kg to mice as an I/P injection. Will someone please check my calculation? (Mw: 130.078 g/mol) Max volume to be administered to mice I/P...

23 June 2024 3,847 1 View

Finding Survey Participants?

Dear Survey Experts, I am currently researching trust and resilience in the supply chain and seeking efficient ways to find survey respondents. In addition to social media and business...

21 June 2024 395 2 View

How to interpret meta-analysis of incidence rates?

Hi! I have a question. I was looking into the research question: does mohs micrographic surgery give less recurrences and metastasis than standard excision in the treatment of cuteanous squamous...

17 June 2024 7,769 5 View

How to functionalise the surface of a PMMA microparticle with amines?

I have porous PMMA microparticles up to 1mm in diameter. I would like to functionalise the surface with amines whilst keeping the particles intact. Chat GTP has recommended ethanolamine or ATPES...

13 June 2024 7,977 1 View

How to avoid metal contamination from a sonicator?

Hi, I have been using a Q-Sonica Q500 Sonicator to disperse samples on nanocellulose and make emulsions. I have notices black dust like particulates settling out in some samples and on the bottom...

12 June 2024 9,737 6 View

How to calculate the conversion of random copolymerization mixture?

I'm working on the RAFT copolymerization of MPC and NIPAm monomer, but I'm not sure how to calculate the total conversion from proton NMR? Is it an average of conversions of two monomers or just...

10 June 2024 7,430 2 View

Can I or you add publications to the page of Adri Jacobs?

Dear ResearchGate, I came across the ResearchGate page of Adri Jacobs, who passed away in 2010. This page contains 25 publication of Adri mainly from the period 2000-2008. One paper (2011) must...

01 June 2024 8,763 1 View

Lifestyles and correlations in cultural tourism?

I am looking for resources in the Netherlands regarding cultural tourism. So what cultural activities correlate, and what lifestyles can be distinguished. Any resource is welcome, articles, factor...

27 May 2024 447 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

Why there is a absence of colonies on the Abx plate for selection after liposome mediated DNA transfer ?

Hi All I am looking to get your opinions, I have perfomed liposome mediated DNA transfer into bacteria, the DNA is a plasmid with msfGFP and when I induce the plasmid I see the fluorescence, and...

16 July 2024 4,698 4 View