some papers perfuse the heart with normal saline 0.9 (50 ml)then 50 ml paraformaldhyde and others use 0.1 M phosphate-buffered saline (PBS; pH 7.4) followed by 4% paraformaldehyde in 0.1 M phosphate buffer (PB; pH 7.4) which is the most correct
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Thanks for support
25 June 2024 4,475 0 View
i make a design topology of mixer For my graduation project working at 28 Ghz on cadence with UMC pdk and get this result S(2,1) 3.5db but when trying to make same topology with another pdk on...
09 June 2024 9,729 3 View
I am trying to form some ligands and some time it necessary to remove the water form by the elimination of it from the reaction .
10 May 2024 8,899 5 View
I'm interested in this paper : A Data Dissemination Algorithm Based On Maximization of Causal Path Entropy in Vehicular Ad Hoc Networks Could you email me this paper?
19 April 2024 2,940 0 View
I need to separate my drug from a sample with activated sludge and residual water
17 April 2024 9,601 2 View
Seeking a protocol to isolate the capping agents from prepared nanoparticles how to characterize them ?
01 April 2024 6,513 3 View
Hi, I just want to ask regarding flow cytometry method for indirect staining of U87 cells (Glioblastoma) as I'm still quite new in using this method. My objective is to observe the expression of...
23 March 2024 8,883 1 View
GROMACS version: 21.0 GROMACS modification: No I complete a 100 ns MD using gromacs v21.0 Can you please provide me a step by step command lines to complete clustering analysis and Free energy...
03 March 2024 2,482 1 View
I am currently working on measuring the zeta potential of nanosheets, but I've encountered a challenge related to the unknown refractive index (RI) of my samples. Since the exact RI of the...
20 February 2024 2,420 3 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
For example, if low-density polyethylene (LDPE) is equilibrated with PCB contaminated marine and freshwater sediments, can LDPE-lipid partition coefficients be used to obtain equilibrium...
28 July 2024 263 1 View
In order to test different water parameters I need to know how to convert conductivity to Salinity and TDS in ppm or ppt. Give me the reference please.
27 July 2024 2,414 2 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.
13 July 2024 7,462 1 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
11 July 2024 7,786 3 View