I am doing the antifungal potential study of seaweed extract. I used four different types of solvents(dichloromethane, methanol, chloroform, aqueous) to extract my sample(seaweed) through soxhlet. After doing the antifungal test, the most effective extract against fungus will proceed with GCMS/HPLC to see the compounds in the extract. However, I have not found method from any article that is using the same extraction method to do HPLC/GCMS. Can I just proceed with the extract that I have prepared to do HPLC/GCMS?
If the compound have antifungal activity, I think you can predict the group of active compound, alkaloid for example. So you can use condition for alkaloid analysis using HPLC.
Or maybe the active compound is terpenoid, you can analyze it using GCMS with suitable condition for terpenoid
Tq Mr. Ashadi for your respond. That means, the procedure for HPLC sample preparation is fixed for every compound regardless of extraction solvent that I used in my research?
you have prepared different solvent extracts, then after doing the antifungal activity you will identify the the active extract. Just to know that which class of compounds are present in that particular extract you may perform simple tests for different classes of compounds. If you want to purify then you may use column chromatography or if you want to use hplc then you can dissolve the substance in any suitable solvent or you can use the solvent of the hplc system.
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