Hi,

generally, tris edta buffer or hepes KOH is ok for extraction of plant proteins. It depends on your purpose as for detergent. If  you only want to do western blotting, it is not necessary to add detergent, because after crude extraction, ER is still in the supernatant. But for other purposes, you should take the effect of detergent on the following experiment into account.

Best,

Thank you so much for your valuable comment. Actually after check expression level by WB, I want to do purification and biological activity assay. In that case what kinds of buffer should I use?

Hello First you tell me.. for what purpose you are using protein homogenization... According to me 50 milimolar sodium phospahte buffer  ph 7.2 or You use very simple 1x PBS... if you want after homogenization you can use sonication.