Yugantak Raj Gupta

4 Questions 15 Answers 0 Followers

Questions related from Yugantak Raj Gupta

What is the most effective buffer technique for optimizing the detection of high molecular weight proteins (500 kDa) in Western blot?

Protein Isolation and Gel Electrophoresis: Total protein from mammalian cells was isolated using RIPA buffer following the manufacturer’s protocol. The proteins were then run on a 3-8%...

09 October 2024 4,695 3 View

Does Realtime Primers Optimization needs Gradient PCR?

Hello All I have a question regarding real-time (RT) Primers Optimization. We usually perform gradient-PCR with standard PCR conditions for primer optimization for qualitative PCR. But for...

06 May 2023 8,892 2 View

Non-specific Bands comming at high Tm. Any suggestion ?

Hello I ran a Gradient-PCR, annealing Temp ranging from 63 to 67. I am getting a faint band at the top of the gel and down to my GOI band. (Image attached). I have tried nearly everything from...

02 May 2023 7,540 2 View

Can anyone suggest why I might be getting less spots than expected with 2-D gel electrophoresis?

I am trying 2-D gel electrophorosis. when I am doing that sample in normal 12% SDS page I am getting good band but in 2-D less spots than expected.... I am using GE helthcare 3-10pH strips. During...

04 May 2015 1,410 18 View