Yugantak Raj Gupta

2 Questions 5 Answers 0 Followers

Questions related from Yugantak Raj Gupta

Does Realtime Primers Optimization needs Gradient PCR?

Hello All I have a question regarding real-time (RT) Primers Optimization. We usually perform gradient-PCR with standard PCR conditions for primer optimization for qualitative PCR. But for...

06 May 2023 8,839 2 View

Non-specific Bands comming at high Tm. Any suggestion ?

Hello I ran a Gradient-PCR, annealing Temp ranging from 63 to 67. I am getting a faint band at the top of the gel and down to my GOI band. (Image attached). I have tried nearly everything from...

02 May 2023 7,473 2 View