Md Reyazul Islam

5 Questions 5 Answers 0 Followers

Questions related from Md Reyazul Islam

What is the minimum concentration of glycoproteins (in total) required for analysis of N-Glycans using MALDI-TOF mass spectrometry?

I have expressed a human glycoprotein in plant ER through a signal sequence and it also contains ER retention signal at the C-terminus. Thus, if this protein contains an N-glycan moiety, it should...

20 December 2018 4,495 3 View

What is the efficient and cheapest protease inhibitor cocktail for preparing total soluble protein from plant cell and tissue extracts?

I am using the Roche cOmplete ULTRA Tablets for extraction of total soluble protein from leaf tissues. Does anybody know any cheaper but efficient one? Any suggestions would be greatly appreciated!

14 December 2018 9,266 4 View

What is the best flexible linker for a fusion protein drug or protein engineering?

I am preparing a fusion protein for the production of protein drug in plant expression system. As I am planning to use several function domains in one construct, therefore I wanna put flexible...

18 November 2015 6,648 4 View

What is the most suitable buffer system for protein extraction from plant tissue/cell?

I wanna extract my expected protein which is targeted to ER in plant system. May I really need to add any detergent such as SDS or triton X100 in extraction buffer? Does PBS buffer is good to use...

13 May 2015 2,628 3 View

Does somebody have a good step-by-step protocol of hemoglobin zymography (PAGE analysis) for assessing hydrolytic activity?

Studying hydrolytic activity on the basis of hemoglobin as a substrate.

04 February 2014 878 0 View