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Questions related from Md Reyazul Islam
I have expressed a human glycoprotein in plant ER through a signal sequence and it also contains ER retention signal at the C-terminus. Thus, if this protein contains an N-glycan moiety, it should...
20 December 2018 4,441 3 View
I am using the Roche cOmplete ULTRA Tablets for extraction of total soluble protein from leaf tissues. Does anybody know any cheaper but efficient one? Any suggestions would be greatly appreciated!
14 December 2018 9,230 4 View
I am preparing a fusion protein for the production of protein drug in plant expression system. As I am planning to use several function domains in one construct, therefore I wanna put flexible...
18 November 2015 6,609 4 View
I wanna extract my expected protein which is targeted to ER in plant system. May I really need to add any detergent such as SDS or triton X100 in extraction buffer? Does PBS buffer is good to use...
13 May 2015 2,585 3 View