I'm planning to perform infiltration in Arabidopsis thaliana plants. I'm undecided about selecting the opine for Agrobacterium strains. What is the most suitable opine for Arabidopsis thaliana?
You can use acetosyringone (100 micro molar). Use DMSO as solvent.
We managed a necropsy on a wild land tortoise that arrived at our faculity hospital due to a traffic accident and was near death. I found small nematodes clustered in its intestines, as depicted...
12 November 2023 3,586 3 View
Dear Colleagues, I want to get some advice from you. Education, Teacher Training etc. Could you please suggest a journal themed, scanned in Q1 index, high impac factor, fast turnaround, free of...
15 November 2022 7,393 6 View
Hello, I am researching into how bacteria communicate. What I've found is that they use quorum-sensing*, and electrical signals**. I've also come across the ability to "speak and listen"***, but I...
03 September 2020 9,416 8 View
Hello, this is yet another urgent question for my assignment: Does the genus Capsicum contain solanine in their stem like other nightshades do? Thank you for your answers in advance!
03 June 2020 4,075 11 View
Hello, I am reading on vaccines in general and I wonder if the beneficial effects of vaccines (as vaccination is the most effective choice when pathogens are to be stopped and prevented) lower the...
27 April 2020 9,103 6 View
Hello, this is an urgent question that I have unfortunately failed to find answers to, I need the information on how often radiation abscopal effect is observed in a typical case, for one of my...
28 March 2020 6,966 4 View
Hello, I use Lumerical/FDTD Solutions. I can create the array of structures on a line. This array will work as a lens to focus when excited to Gaussian source. However, I want this lens array to...
16 October 2019 2,147 2 View
The first question is If i can use PBR for it how can calculate for Na2Ti3O7? The second question is When a higher valency cation, for example Nb5+, substitutes Ti4+ in rutile lattice, it is...
18 August 2018 4,376 2 View
I am working on Cu2ZnSnSe4 semiconductor thin film on glass substrate. The thickness of absorber is around 650 nm-720 nm. I roughened the back side of the substrate to suppress the effect of back...
01 January 2017 1,247 2 View
Is there anyone with experience on the etching process of ZnO from the Cu2ZnSnSe4 absorber layer? (the structure is: ZnO/CZTSe/ZnSe/Mo)
06 April 2015 6,610 4 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View
Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...
25 June 2024 9,151 6 View
Introduction: I am conducting an experiment to investigate the root phenotypes of Arabidopsis Col-0 plants grown under phosphate deficiency conditions. Here, I describe my current methodology and...
19 June 2024 8,774 3 View
Should I store in -20C or -80C? And do I need to store them in buffer? How long can I store?
16 June 2024 7,309 5 View
I am working on a paper for my bioinformatics class, where we are running an analysis of transcriptomics on A. thaliana col-0 about the difference in gene expression in the temperatures of 21 and...
12 June 2024 8,822 1 View
Hi all, I have been facing problems with colony pcr for agrobacterium colonies. I did site directed mutagenesis, cloned to pcambia, sequenced and confirmed and then transformed to Agrobacterium....
02 June 2024 6,517 5 View
Hello all. I transformed some Agrobacterium competent cells by electroporation and performed colony PCR to see the positive transformants. However, I performed the glycerol stock after 10 days of...
26 May 2024 9,304 3 View
Hi everyone, I'm having trouble with my hydroponic cultures for Arabidopsis thaliana, since lately I've started having algae contamination on them. I have tried using several sterilization...
21 May 2024 4,840 1 View
I am confused regarding the desired edit efficiency of PEP2 for transversion on rice plants using Agrobacterium.
21 May 2024 4,332 0 View