How does Anti-retroviral therapy effect cryptococcal meningitis?

I have read that early administration of ART in cases effected with cryptococcal meningitis lead to a higher level of mortality but unfortunately could not get a clear understanding about the...

31 December 2018 7,040 0 View

I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal?

I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal or a potent antifungal drug candidate? Is there any...

13 October 2018 7,718 3 View

I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal?

I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal or a potent antifungal drug candidate? Is there any...

09 October 2018 9,955 4 View

Need a protocol for PI and Dapi staining to identify dead cells for confocal microscopy?

I am looking for a protocol to stain yeast cells with PI and DAPI in order to identify dead cells with confocal microscopy.

10 August 2018 2,365 1 View

Need a protocol for PI and Dapi staining to identify dead cells for confocal microscopy?

I am looking for a protocol to stain yeast cells with PI and DAPI in order to identify dead cells with confocal microscopy.

07 August 2018 4,631 1 View

How to decide an antimicrobial's concentrations to be used to check susceptibilities if it has not yet been used against the organism of interest?

I am testing plant product against a yeast and want to determine the minimum inhibitory concentration of the same against the fungi. It has not yet been used against the said yeast. What...

06 July 2018 1,111 1 View

Can any one tell me about efficient fluorescent plasma membrane markers for yeast cell?

I want to visualize the effect of a drug on the plasma membrane of the yeast cell. What are the fluorescent markers that I can employ for this?

01 February 2018 4,937 2 View

What might be the probable reason of Malondialdehyde level going down in case of fungal infection and rising after treatment comparing with control?

I am working on fungal infections in rodents. I am interested in knowing that what might be the probable reason of lowering of the MDA levels in case of infected groups and increase of the same...

09 October 2017 2,389 0 View

I need to install GROMACS on windows 10, 64 bit system. Can anyone help me with a step by step tutorial?

08 September 2017 1,011 16 View

Why are Minimum inhibitory concentration indicated as MIC 50 or MIC 80 or some times MIC 90?

Which one should be determined and what is the basis of selection? Minimum inhibitory concentration against fungi

05 June 2015 9,011 23 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View