Protein sequencing
When I am importing the orbit file created using STK into OPNET, I am getting the following error: Unable to import STK orbit file (C:\Users....). How can I fix this?
11 March 2023 5,020 2 View
I have read that early administration of ART in cases effected with cryptococcal meningitis lead to a higher level of mortality but unfortunately could not get a clear understanding about the...
05 January 2019 8,688 0 View
31 December 2018 7,124 0 View
I am working on a natural compound which is active against cryptococcous hving an MIC around 5.5 mg/ml. Can it be considered as an antifungal or a potent antifungal drug candidate? Is there any...
13 October 2018 7,793 3 View
09 October 2018 10,051 4 View
I am looking for a protocol to stain yeast cells with PI and DAPI in order to identify dead cells with confocal microscopy.
10 August 2018 2,451 1 View
07 August 2018 4,719 1 View
I am testing plant product against a yeast and want to determine the minimum inhibitory concentration of the same against the fungi. It has not yet been used against the said yeast. What...
06 July 2018 1,181 1 View
I want to visualize the effect of a drug on the plasma membrane of the yeast cell. What are the fluorescent markers that I can employ for this?
01 February 2018 5,042 2 View
I am working on fungal infections in rodents. I am interested in knowing that what might be the probable reason of lowering of the MDA levels in case of infected groups and increase of the same...
13 October 2017 2,250 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
