I am trying to study the solubility of a very hydrophobic drug in various lipids that are solids at room temperature. Once the drug is assumed to have acquired saturation solubility in that phase (obviously at temp above the melting point of solid lipid) how easy is it to analyze the drug in the lipid matrix by HPLC? Is there a general sample clean up procedure that one could follow to modify for the drug in our particular case? Would SPE be recommended or some other analytical tool? I would appreciate if someone could share their experience in this regard. Thanks and warm regards
Manish
Dear Dr Issar!
This is not my field but I have noted that Waters has a rather good account on SPE on their home page:
http://www.waters.com/waters/en_SE/Beginner%27s-Guide-to-SPE-%5BSolid-Phase-Extraction%5D/nav.htm?locale=en_SE&cid=134721476
Have a look and you may find some hints that could help you.
Best wishes
Sure F.
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