Orbitrap Fusion FT keep getting disconnected?

Hi, I been having a problem with the Orbitrap Fusion for quite a while and it has been getting more and more frequent, which is very frusttrating. The fusion FT would get disconnected suddenly...

22 January 2021 1,235 4 View

What is the basis set to optimize ZnO using GaussView for DFT calculation study?

Greetings, I have a bit of problem in setup the basis set of my DFT study for ZnO.. As you can see in the image attached below, I use B3LYP 6-31G (d,p) for geometrical opt. of ZnO.. But sadly, I...

13 January 2021 7,508 4 View

How does titanium dioxide (TiO2) as a coating's component enhance the coating performance in terms of anticorrosion??

Greetings, Recently, I was asked by a researcher how does TiO2 enhance the coatings polymeric structure making it able to reduce corrosion reaction by preventing the penetration of aggressive...

02 January 2021 5,257 5 View

What are the suitable amplitude of sinusoidal perturbation signal to analyzed stainless steel 316L?

Greetings researchers, I want to ask for your suggestion in regards to what is the suitable amplitude of sinusoidal perturbation signal to analyzed the conditions of bare and coated stainless...

07 December 2020 7,699 7 View

How to improve DNA extraction using TRIzol and chloroform?

02 December 2020 1,919 1 View

What is the solubility of crude extract of plants in water?

Greetings all, To my limited understanding, the crude extract of plants obtained using solvents such as ethanol and methanol are usually insoluble in water. Is this correct? If it is soluble in...

02 December 2020 1,024 6 View

Does the concentration of the fluorescent compound affect the fluorescent reading on a 96-well plate?

I am trying to detect the fluorescent level of the extracted RNA sample on a 96-well plate. After extraction, I dilute the RNA sample in 20ul solvent. Can I load the 20ul RNA sample directly to...

30 November 2020 2,616 2 View

Any available international research grant in this COVID-19 year?

Dear researchers, I am a researcher from a university in Malaysia. I know that we all are having a tough situation in this pandemic year. I just posted this to know if there is any possible...

24 November 2020 8,759 3 View

In your opinion, what are the mechanism performed by corrosion inhibitor in a coating for corrosive medium?

Greetings researchers, There are quite a number of studies has been performed to analyze the effects of corrosion inhibitor when incorporated in a coating (epoxy, alkyd and etc) for the...

23 November 2020 6,622 10 View

For a marine coating, how much does its mechanical strength needs to be?

Greetings to all researchers, First and foremost, I would like thank all of you for answering this question. So, my question is quite general I guess, for a standard marine coating with good...

08 November 2020 8,880 4 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 1,808 1 View