I would like to isolate DNA-RNA virus for metagenomic analysis and theoretically I've just purify my samples for virus with some treatment (involved PEG). I don't want to distinguish between RNA virus or DNA virus because I'm interested in a general analysis. What kind of Kit or protocol is advidsable for my assay? I think that any kit could be useful if I omit the RNAse or DNAse step. I expect that expert people can help me. Thanks beforehand.
Hi.....
I don't have any information about such kit to isolate DNA-RNA virus. What is your exact research objective, what kind of study you want to carry out further will be helpful to answer your question? But by general observation, I can suggest you go for direct DNA isolation or RNA isolation from an environmental sample and depends on phylogenetic marker or gene specific primers you can be able to study viral diversity. However many of the times while isolating DNA from environmental sample both DNA and RNA will get extracted if you don,t treat your preparation with RNase. The method called molecular troughing will help to separate such DNA and RNA of one sample simultaneously and can be used for further analysis.
Thank you for your interest. I didn't see the notification about this answer, sorry about the delay. The analysis is about high throughput sequencing for virus diversity in environmental samples.
I obtained the virus phase by PEG treatment and I verified the presence of virus by microscopy (TEM). Now, I would like to isolate DNA and RNA for downstream applications.
I wonder what is the best kit, I've seen several of QIAGEN like QIAamp MinElute® Spin Kit, QIAamp MinElute Vacuum Kit and QIAamp UltraSens® Virus Kit.
I would like if someone has experience in the use of any of these kit to recommended me what is the best choice for my assay. I was reading about the differences and It supposed that is about the sample origin and concentration. But my aim is to obtain the DNA and RNA. My question is: What is the better Kit or protocol to obtain DNA and RNA for virus? It would be enough any kit (adapted to the sample features) if I don't use RNAse and DNAse?
I expect to have been clearer.
Thanks beforehand.
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