What is the molar excitation coefficient for collagen?

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Can we estimate neurotransmitter (dopamine and GABA) from cell culture media after activating neurons? Is there any methods other than HPLC?

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02 March 2015 10,120 7 View

Has anyone had any experience with one of the following peptide suppliers?

I want to buy custom peptides from the following suppliers but I am looking for some your input, to know whether anyone has used them before. Custom peptide suppliers that I am looking at...

02 March 2014 513 4 View

Can I measure the concentration of H2O2 in cell culture media (pink colour) at 240nm?

I doubt that the coloured solution will interfere at 240nm. Regardless, I have blanked the medium.

10 November 2013 8,529 9 View

i would like to get guidance under eminent professionals working on cancer in chennai, India.

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08 September 2011 427 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

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06 August 2024 1,989 3 View

Low-yield gel extraction problem?

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Transfection in HEK293T cells?

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31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

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What is the relationship between protein structure and N or C terminal tagging choosing?

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28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

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How does the Retention time effect the Biogas Production?

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Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

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EDC carbodiimide and NHS easter 's half life among different pH?

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Does post-translational protein modification cause devisions on observed pI verses calculated pI?

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Vinoth Sankarapani

I Found this article , hope it helps http://scholarsresearchlibrary.com/APR-vol2-iss4/APR-2011-2-4-50-59.pdf

Marcia Moss

There is a program on the internet, where you put the sequence of the protein in, and it gives you the extinction coefficient. I forgot the name but you can google it. It might be under EXPASY. Maybe others can comment.