What is the minimum viral load that can be detected by TEM or SEM?

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Shivani Raval

Electron microscopy usually requires a minimum concentration of particles for reliable identification of microbes. For viruses, this is between 10^5 to 10^6 virus particles/mL. With filtration techniques, both TEM and SEM of viruses can be carried out with as little as 5000 particles per sample.

Melvin Hayden

Great question to pose and great answer by S Raval.

Melvin R Hayden, MD

University of Missouri School of Medicine

Columbia, Missouri USA

Muhammad Yousuf

A very important question. Following articles are very helpful to fathom the answer by use of TEM and SEM:

1. Article The Role of Electron Microscopy in the Rapid Diagnosis of Vi...

2. Article Electron Microscopy Methods for Virus Diagnosis and High Res...

3. Article Applications of transmission electron microscopy to virus de...

4. Article Virus quantitation by transmission electron microscopy, TCID...

5. Article Accurate virus quantitation using a Scanning Transmission El...