Hi,
I'm working with human retinal pigmented epithelium cells and I'm wondering if anyone working with these cell lines has ever drawn a killing curve with G418 antibiotic?
I came across with the minimum concentration of 0.4 mg/ml to kill over 90% of these cells. However, I observed this effect within 10 days.
Within 4 days this number changed to 0.8 mg/ml.
Which one do I need to choose if I'm applying a selection marker?
Thanks in advance for any help.
Usually you want to look at the concentration that gives massive cell death within 3 days and that kills all cells within 1-2 weeks. And then you choose the concentration that is just lower to do your selection. So let's say you tested 0.5, 0.6, 0.7, 0.8, etc. Based on your results I would choose 0.7 to perform selection, and then lower it to about half once the selection is complete, to maintain the cell line.
Hope that helps.
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