Nafiseh Nafissi

3 Questions 11 Answers 0 Followers

Questions related from Nafiseh Nafissi

Immunofluorescence staining protocol: can I leave fixed cells in antibody solution for 4 days at fridge?

I'm running Immunofluorescence imaging today, and I added the antibody. Cells are i at 37 incubator for four hours. I wont be able to finish imaging today due to the number of samples. Can I leave...

09 October 2015 6,922 5 View

What is the minimum inhibitory concentration of G418 in ARPE-19 cells?

Hi, I'm working with human retinal pigmented epithelium cells and I'm wondering if anyone working with these cell lines has ever drawn a killing curve with G418 antibiotic? I came across with the...

11 November 2014 2,158 2 View

What is the minimum inhibitory concentration of G418 in retinoblastoma (Y79) cells?

I'm running a site-specific insertion experiment on retinoblastoma (Y79) cells and I'm wondering is anyone has experience working with this cell line?

11 November 2014 5,400 4 View