I need to test the reaction to Pilocarpine of some mutant mice and need to know the exact maximum dose of Pilocarpine to which wild type animals do not respond.
200mg/Kg body weight is lowest dose at which occasionally seizure can be seen. Increasing dose from 300mg/kg induces seizure which can be clearly noticed...Here is supporting article for your reference ..
http://ac.els-cdn.com/000689938490177X/1-s2.0-000689938490177X-main.pdf?_tid=a63e056a-0ccd-11e4-bab1-00000aacb35f&acdnat=1405503992_78806855626bb062299b92fea6d29157
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518220/ This is a great [free] review on the pilocarpine model. We've used 300 mg/kg in adult male rats but it is highly age- and model-dependent.
Pilocarpine hydrochloride (soluble in saline) is a muscarinic agonist commonly used to induce seizures. Muscarinic acetylcholine receptors (mAChRs) subtype M1 mediates seizure induced by an i.p. administration of 200 mg/kg or 300 mg/kg of pilocarpine in mice. https://doi.org/10.1073/pnas.94.24.13311
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