hi Shirima..

use agilent Bioanalyzer its one of the best machine for sensitive quantification and the bonus is that u get to know the quality of ur RNA also.

Gud Luck !!

You need an equivalent standard for your experiment. Such an RNA standard isn't cheap but you need it for absolute quantification.

The most technologically advanced (and probably most reliable) is Digital PCR. In this approach you make huge dilutions of your template. Then you (mean - special instrument, available from Bio-Rad or LifeTech) make microreactors in such a way that in every nicroreactor will be much less than one copy of the template. Then you just count positive pcr events and calculate quantity of virus based upon dilutions you've performed.

I attach paper about absolute HIV quant-n.

Cheers!

Thanks Santosh, Nadine and Stephan for your inputs.