Stepan V Toshchakov

4 Questions 17 Answers 0 Followers

Questions related from Stepan V Toshchakov

De novo assembly of bacteria cultivated in enrichment cultures?

I'm trying to assemble a genome of halobacteria from the DNA sample obtained from enrichment culture (culture with media, optimized for growth of 1 species, but it obviously contains a mix of...

11 November 2013 9,261 3 View

Can someone suggest any analogs of Epicenter's Plasmid-Safe ATP-dependent DNAse?

We'd like to use it for mate-paired library construction, but unfortunately there is no way to order it in Russia.

08 August 2013 9,286 4 View

Where is the point to stop with in-silico assembly?

We're assembling de novo bacterial genomes with ILMN system. After the first run, we have N50 about 120-150 kb and are now trying different assemblers to orient configs relative to each other and...

02 February 2013 4,561 1 View

Reptile NGS read correction tool

I've tried to use Reprile error correction tool ( http://aluru-sun.ece.iastate.edu/doku.php?id=reptile ) for my MiSeq reads, but unfortunately this soft cannot read my fastq file. Do you know if...

01 January 2013 2,108 1 View