Santosh P

4 Questions 6 Answers 0 Followers

Questions related from Santosh P

What is a low cost and effective method for native bacterial cell lysis protein purification?

I am looking for an effective and rapid bacterial BL21(DE3) cell lysis for protein purification. I am not interested in using the commercial reagents such as BugBuster reagent and others. Please...

04 April 2014 9,665 8 View

What is the easiest and a most rapid method of His Tag removal?

I have cloned a gene in pET28a with a protein size of 47KDa, I am obtaining fairly good yield after induction and purification using the Ni-NTA Agarose (Qiagen). I would like to know the easiest...

04 April 2014 4,210 5 View

Optimization of Pichia Expression?

I am expressing a plant gene in Pichia and have observed a faint band of expected size on SDS-PAGE. However the yield is low (Approx 7 micro gram for 25mL culture volume). Please suggest me the...

02 February 2014 8,434 1 View

How to choose the most suitable genes for qRT-PCR after having done a transcriptome analysis?

I have done a transcriptome analysis of a medicinal plant and have got few genes for an important metabolite. Now my question is how to choose the right candidate genes for the qRT-PCR?

01 January 2014 6,368 6 View