I would like to purchase a digital PCR system and I am not sure between the two which one I should go for. I would like to use the technology to quantify virus titre in crops.
I am looking for a reliable supplier in East Africa for the "TOPO® TA Cloning® Kit, Dual Promoter, with One Shot® TOP10 chemically competent E. coli cells" (Catalog number: K4600-40). what is the...
11 December 2014 8,638 0 View
I am setting up a new StepOne Real-Time PCR system (48 well) that came with a laptop computer with all the installation completed. The RNase P Experiment "Failed". There were virtually no...
08 September 2014 8,508 4 View
I would like to monitor gene expression in virus infected plants. Is there any cost effective but robust method for this?
06 July 2014 9,630 2 View
I would like to study the interaction between cassava viruses and a sort of cassava genotypes. I would like to do this by measuring virus concentration. Is it right to call this virus...
05 June 2014 5,875 9 View
I would like to perform absolute quantification of RNA viruses in plant tissue and was wondering if approaching this through in vitro transcription makes sense.
31 December 2013 672 6 View
I am trying to design primers for a tomato virus isolate and find that I need to allow two consecutive ambiguous nucleotides for the last two bases at the 3' end.
10 November 2013 2,254 4 View
Absolute quantification of (plant infecting) RNA viruses
09 October 2012 9,036 4 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...
06 August 2024 9,986 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I am seeking experimental or applicable data for the liner (LLDPE) interface in FLAC3D numerical modeling of a large stockpile. Could you please recommend suitable references? The preferred data...
05 August 2024 3,665 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
What are the biological importance of bacteria and the role of microbes to improve crop productivity and soil health and role of bacteria as decomposers in cycling nutrients?
31 July 2024 3,479 5 View
What is the role of microorganisms in soil fertility and crop production cycles and role of microorganisms in mineral transformation?
31 July 2024 8,483 8 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View