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Questions related from Rudolph Shirima
I would like to purchase a digital PCR system and I am not sure between the two which one I should go for. I would like to use the technology to quantify virus titre in crops.
09 September 2016 8,974 0 View
I am looking for a reliable supplier in East Africa for the "TOPO® TA Cloning® Kit, Dual Promoter, with One Shot® TOP10 chemically competent E. coli cells" (Catalog number: K4600-40). what is the...
12 December 2014 8,618 0 View
I am setting up a new StepOne Real-Time PCR system (48 well) that came with a laptop computer with all the installation completed. The RNase P Experiment "Failed". There were virtually no...
09 September 2014 8,481 4 View
I would like to monitor gene expression in virus infected plants. Is there any cost effective but robust method for this?
07 July 2014 9,608 2 View
I would like to study the interaction between cassava viruses and a sort of cassava genotypes. I would like to do this by measuring virus concentration. Is it right to call this virus...
06 June 2014 5,849 9 View
I would like to perform absolute quantification of RNA viruses in plant tissue and was wondering if approaching this through in vitro transcription makes sense.
01 January 2014 650 6 View
I am trying to design primers for a tomato virus isolate and find that I need to allow two consecutive ambiguous nucleotides for the last two bases at the 3' end.
11 November 2013 2,230 4 View
Absolute quantification of (plant infecting) RNA viruses
10 October 2012 9,013 4 View