In diabetes vascular occlusion is present, which interfere in the healing process and delay the wound healing. I want to to know that why which method (any special staining protocol) it is possible to distinguish the normal and occluded vessels.
We have evaluated the wound healing potentials of different compounds at particular concentrations in laboratory animals. Now, we want to move in the direction of drug development for some topical...
31 December 2019 7,260 1 View
We want to determine the antioxidant effects of our prepared formulation of nanoparticles by DPPH method. Our formulation is having some col and different excipients/vehicles. Please also tell how...
09 October 2019 9,387 2 View
I am interested in assessing the expression of some growth factor (e.g. TGF-beta 1) or other marker (e.g. PCNA, CD31). I want to know the difference in term of significance for assessing the same...
02 March 2018 6,841 3 View
I want to measure the % wound contraction from the data of wound area by using Wilson's formula. Can anyone provide me the formula and reference for this formula?
05 June 2016 6,434 0 View
I want to mix two and more than 2 herbal drugs for the preparation of herbal formulation. Can anyone suggest or provide me the PDF file of books on herbal dose selection, their standardization in...
04 May 2016 4,409 0 View
I am interested and doing research in the field of wound healing and want to explore this area for the development of wound healing formulations faster healing. Can anyone suggest or provide PDF...
04 May 2016 6,964 1 View
I am using DMSO as a vehicle for dissolving the drug for the the topical application on wounds. Dose anyone have observed the adverse effect of DMSO on wound healing or other side effects?. What...
03 April 2016 4,246 5 View
I want to disolve drugs in DMSO and want to apply them topically on wounds, but some researchers have pointed me that it is cytotoxic even at lower concentration i.e. 10% or more. Can anyone...
03 April 2016 1,379 8 View
Citrate buffer is used for dissolving the streptozotocin for diabetes induction. Is the tri-sodium citrate used for tie preparation of citrate buffer?
03 April 2016 8,544 2 View
I have prepared some plant extracts in different solvents and among them methanolic extracts are showing best in-vitro results for antioxidant potentials. Now, I want to feed or administer these...
01 February 2016 9,935 4 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...
20 July 2024 7,922 2 View
These datasets will be used in the training of machine learning algorithms. Does anyone know any available data?"
17 July 2024 6,519 3 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
I'm simulating a magnetohydrodynamic problem of blood flow using lattice Boltzmann method in lattice units and I need to convert the magnetic conductivity to lattice units. The physical value is...
07 July 2024 5,243 0 View