If you're using a kit such as PicoGreen Quant-it to get your DNA, the secretion itself doesn't need to be normalized to that, your secretion can be normalized to the insulin content of the islets for each condition as follows:

(secreted insulin/(secreted insulin + cell fraction insulin))*100

That gives you insulin secreted as a % of the total insulin content

I've also seen people use normalized insulin secretion, which is the same thing, but then divide your high/stimulated condition by your low condition to get normalized (i.e. set low glucose equal to 1)

If you're interested in knowing the insulin content of the islets normalized to the DNA content, you should use the following:

(insulin secreted + cell fraction insulin) / DNA content

Hope this helps!

Sydney,

We do in the same way as Dr. Ceasrine commented, using picogreen to quantify DNA and, then, divide (insulin secreted + cell fraction insulin) / DNA content.