Besides RAPD marker you may use ISSR, Scot, SRAP marker systems, which will give you the appropriate result.

Thank you for your answer Dr. Ashok Kumar Bishoyi . Many markers can be used. But because the number of explants is high, and on the other hand, the RAPD marker is an easy and inexpensive technique, using this method can be reliable, so that I do not need to use another marker?

Thank you very much Dr. Kapiel for the chapter you sent me.

Hello

I agree with Dr. Kapiel about "Date Palm Biotechnology" book.

Additionaly ISSR marker provide a useful technique in the assessment of true to typing of date palm as well as in their genetic characterization, allowing the differentiation of cultivars with a relatively low number of primers, even those corresponding to a same family (Ahmed et al., 2012).

This paper help you. Good luck

Dear Dr. Ibrahim

Thanks for giving me a chapter of your book.

u can also read this

Dear Dr. Bhati

I read your paper that assesment somaclonal variation in samples prepare by callus induction but do you have any investigation for RAPD marker on in vitro planlets via somatic micropropagation of date palm?

Thanks for your attention

Dear Dr. Bhati

Thank you for your valuable article.

Dear Dr. Roughani

The explant type and regeneration method (direct or indirect) are not very important for the study of somaclonal variation using RAPD markers.

Dear Dr. Miri

Thanks for explanation

Both SRAP and SCoT markers are better compared to RAPD markers for genetic fidelity test. Further, you may have to select randomly 10 plants in every 1000 plants for this type of testing . You can read the following article.

Field Performance and Genetic Fidelity of Micropropagated Plants of Coffea canephora (Pierre ex A. Froehner)

published Online:| DOI: https://doi.org/10.1515/biol-2017-0001

Thank you very much Dr. Mishra for your valuable opinion. I also agree with these markers. SRAP markers are simple, inexpensive, and effective for producing genome-wide fragments with high reproducibility and versatility, which are used to amplify coding regions of DNA with primers targeting open reading frames. Also, SCoT markers were generally reproducible.

In working with thousnds datepalm plantation in kutch, what i have understood is many at times most markers mentioned above can give you false positives, i am not saying they are not good research but can be mostly non repeatable, i would rather prefer you using these markers and run it in DGGE ya TTGE, These both methods i have tried and found the resolution of markers is increased to 100x

Thank you very much Dr. Rajr. Do you have a paper on the study of somaclonal variation or genetic diversity of date palm using these markers?