I want to do Lipid peroxidation assay according Ohkawa et al 1979. I want to prepare standard curve using tetra-methoxy-propane (TMP). Whether, I have to prepare the standard curve directly by taking OD of different concentrations of TMP or I have to go though the entire procedure with TMP which I am using for the homoginate samples?
Kindly help me in this regard.
Just use malondealdehyde (MDA) instead of samples at different concentrations and measure the optical density at 532 nm to draw your cure.
The best method is to use MP-Biotek SafTest kit.These kits will come with calibrators from lower range to higher range by which you can build a standard curve. We use this kit extensively for our lipid per oxidation in fish oils.
For standard curve preparation, use different concentrations of MDA standard. In kits, MDA is provided as an acetal as the aldehyde is not stable. During the acid incubation step, the TMOP is hydrolyzed at 45C to generate MDA
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