I am using MDA kit to measure Lipid Peroxidation in tissues. Did anyone ever used it?
I have 2 questions to ask:
Q1) Shall I use fresh or frozen tissue, which is better? I already have frozen ones.
Q2) Which is recommended to use fluorometric or colorimetric assay, since both are described in the protocol.
Thanks.
Use fresh tissues as frozen samples will undergo lipid oxidation (freezer burn) which will generate MDA unless purged with argon.
The fluorometric variant can detect smaller quantities. Depends what equipment you have and how much sample.
Hi Sham, i will suggest fresh tissue or frozen you can use but it should be freezed on -80oC. MDA can be produced by enzyamtic or non enzymatic reaction. MDA react with TBA and produces pink colour that can be detected by colorimetric spectrometer.
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