What is the problem with my siRNA transfection?

I tried a pool of 3 siRNA transfection in Hela cells using RNAiMAX but all of of them failed. 100nM conc siRNA and opti-mem were used at 40~50% cell confluency and then 6 hour later, added DMEM...

02 September 2020 9,096 4 View

Why is methylene blue commonly used to measure an active surface area of nanoparticles?

Among the dyes, why is methylene blue commonly used to measure an active surface area of nanoparticles(especially graphene derivatives)? I try to find the reason, but I cannot find it. Could you...

11 March 2020 2,963 4 View

How can I increase the yield of this compound?

Hi guys, I want to synthesize the compound in the below picture. (Ref from Chem. Commun., 2017,53, 3189-3192 ) Its synthetic procedure seems gabriel synthesis using...

06 March 2018 6,385 3 View

Why does DMSO show quintet in 1H NMR?

Hi guys, I learned that dmso peak in 1H NMR caused by proton exchange. So the peak at 2.5 ppm indicates dmso-d5 not d6 Also the dmso which have more exchanged proton like d4 or others can be shown...

04 March 2018 8,655 3 View

Why brine(saturated NaCl solution) is better than water in extratction?

Hi, I've learned extraction using brine. The reason that I learned was increasing polarity and removing water in organic phase. Then, how this can happen and why brine is better than pure...

10 February 2018 5,516 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

I need to accelerate corrosion in steel parts that are used in a salt-water environment. Is there a technique for doing so?

I have a structure that employs d-ring swiveling tie-downs that corrode during use and become "frozen". I have sample swivel assemblies that I wish to corrode to see if I can free them using one...

01 March 2021 3,739 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View