What is the maximum absorbance of OD at 680 nm for algal growth?

20 December 2022 1 2K Report

Hello everyone,

I would like to ask what is the maximum value of absorbance on the spectrophotometer when I measure the OD of the algal growth at 680 nm? in all my papers, it didn't exceed 1, but the absorbance may reach 4 in other papers, so I confuse is that possible?

I will be appreciated if you helped me to understand this issue.

Thanks

Yi Cui

Hi, the OD value is related to the cell number in the culture. Usually, there is a linear relation between these two values. If your culture is too dense, it is possible that the OD value may exceed 2 and up to 4.

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