What is the maximal time period for viral (CCHFV) RNA to remain intact from the field collection of ticks to molecular lab under freezing conditions?

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Mohammad Djaefar Moemenbellah-Fard

Hi! Thanks for your comments.

Best regards;

Yours: Moemenbellah-Fard

Barry Atkinson

For detection (PCR, sequencing etc) it will be fine for years at -20C and indefinitely at -80C.

For isolation, it is best to process material immediately. If this is not possible then storage at -80C should be fine but I wouldn't recommend -20C (that said it has worked previously)

All the best

Barry

Thanks Barry. That's very kind of you. Best regards. Yours: Moemen