Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

How to remove straight line from mosaic Landsat image?

I have used mosaic to new raster tool to join two aerial image from Landsat 8 of different path. However, raster files had color variation and after mosaic I got a straight line running from...

22 December 2020 4,640 4 View

Can anyone please give me some good suggestions for my undergraduate mechanical engineering thesis?

Our group need to start giving topics on which one should we focus on regarding non renewable resources. I've looking these past 2months but nothing comes to mind.

27 November 2020 4,414 3 View

Can someone help with variable selection for logistic regression while considering missing data?

I have a data set with quite a lot of missing values, more in some variables than others. I performed bivariate analyses and I want to select variables for multivariate analysis. I understand that...

25 October 2020 3,613 3 View

To find maximum compressive stress in concrete using hognested model numerical?

In a reinforced concrete beam depth id 640mm.Depth of neutral axis is 0.43363d.Determine max compressive stress in concrete using hognested model when strain in steel reaches0.00284.Solve

20 October 2020 3,303 2 View

What analysis will be best suited to analyze my likert type questionnaire with both likert item and likert scale type Data in SPSS?

https://forms.gle/METKBvvq6n6eFMLb6 Above is a link to my questionnaire. It has three objectives to evaluate. 1) From Page 2-6 the questionnaire ask the stress factors that effects the...

05 September 2020 3,960 3 View

How long time should we wait for editor decision on a manuscript?

Some editors keep a paper for a long time, more than 6 months or a year, without a decision. So I ask how a paper takes a long time like that for the review process and What the best way to...

13 August 2020 5,797 3 View

Can antibodies that come in serum format be used directly in ChIP, or should they be purified before use?

Hello everyone, I am performing ChIP in plant tissues. I bought a ChIP grade antibody from a company but it came in serum format (unpurified). My question is, do I need to purify it first or do I...

10 August 2020 4,545 2 View

Interpreting MICOM Results in SMART PLS?

Hi, What if in the results of MICOM, one out of six constructs does not have compositional invariance (step2). however, for all constructs the difference in means and log variance is...

08 April 2020 1,545 3 View

How to mine the TCGA Database for RNA sequencing?

Anyone with advice on how to mine data from TCGA Database. I am interested in RNA seq data, i have a gene of interest i to analyze but i cannot seem to locate where to indicate the gene i am...

13 November 2019 5,262 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Hello house, is it necessary to compute Content Validity Ratio (CVR) for an adapted questionnaire pls?

What should one do if he obtains conflicting results between Content Validity Index (CVI) and CVR for items in a quetionnare. For example based on the CVI obtained the items are acceptable but the...

25 February 2021 7,325 2 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Is it correct to identify an air mouvement by the Venturi effect in buildings ?

Knowing that the Venturi effect is used most in tubes (according to my basic knowledge in physics) to increase the speed of air or liquids by reducing the tube volume, my question is if we have...

17 February 2021 1,260 1 View