What is the main difference between HepG2 and HepG2/C3A cell line in HEV transfection??

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Any explanations or reasons for this sds page image?

This is third time I run this sds page 15% and get the same result current 120 volt 80 All ph of buffers adjusted sample dialysed but suddenly no band appears again. Thanks in advanc.

09 October 2018 9,469 3 View

Which kit is better for extraction of RN??A virus from cell culture 1- viral rna and DNA from blood OR 2- total rna from tissue?

Which kit is better for extraction of RNA virus from cell culture 1- viral rna and DNA from blood OR 2- total rna from tissue

06 July 2018 1,222 8 View

Removing polysaccharide from mushroom without affecting protein content??

I'm trying to extract certmain protein from mushroom by ammonium sulphate once and with ethanol another time but the presence of polysaccharide affects my protein activity so is there is a method...

05 June 2018 3,234 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

E-commerce businesses, COVID-19 and Entrepreneurial Strategies (innovation, proactiveness and risk-taking)?

Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey

05 August 2024 5,332 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View