Removing polysaccharide from mushroom without affecting protein content??

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Any explanations or reasons for this sds page image?

This is third time I run this sds page 15% and get the same result current 120 volt 80 All ph of buffers adjusted sample dialysed but suddenly no band appears again. Thanks in advanc.

09 October 2018 9,386 3 View

What is the main difference between HepG2 and HepG2/C3A cell line in HEV transfection??

kindly I would like to know the difference between HepG2 and HepG2/C3A cell line in HEV transfection and if any one try to propagate HEV in HepG2 thanks in advance

06 July 2018 2,403 3 View

Which kit is better for extraction of RN??A virus from cell culture 1- viral rna and DNA from blood OR 2- total rna from tissue?

Which kit is better for extraction of RNA virus from cell culture 1- viral rna and DNA from blood OR 2- total rna from tissue

06 July 2018 1,125 8 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Hayat Essalmani

You use the dialysis beads (membrane) with porosity suitable on the size of your protein

Yehia A.-G. Mahmoud

I see that you make fractionation with ammonium sulfate different saturation percentage and find out your major protein fractions.This will be more practicable

Yousra El-maradny

Thanks a lot for your answer

I'll try dialysis and different fractions of ammonium sulfate