What is the reason of double imaginary frequency?

Hi, i have made hydrolysis of peptide from small protein to learn Computational Chemistry basics. While i try to find Transition state of hydrolysis, i saw double imaginary frequency but one of...

31 December 2018 822 3 View

How the primary cell cultures can be quiescence cell?

I couldn't find a sufficiently detailed resource. This may be a very simple question for the social environment. These cells when I isolate cells from a particular tissue; Could it be from...

09 October 2018 3,543 4 View

While protein is precipitated, will structure be disrupted?

When we use salts for precipitation of proteins for seperation process. Salting out were made by salts but if we block interactions of water and proteins; protein's structure have to be destroyed...

02 March 2018 9,158 3 View

Can be possible the dna binding domain's binding to two or more different DNA sequence in different enhancers?

Can be possible the transcription factor's dna binding domain's binding to two or more different DNA sequence in different(or also same) enhancers? Or there is no exception, it must be specific...

01 February 2018 2,723 3 View

How change dipole moment of this N terminal?

When the aminoacid environment were changed with adding OH or etc., Aminoacid N terminal were affected by H amount and in determined pKa, they loose hydrogen from N terminal. NH3 goes Nh2. My...

11 December 2017 8,074 3 View

How we know the transformation occuring when the blue white screening is made?

Books say 3 possibilities for this situation as insert within Lacz, insert outside Lacz, no inserted vectors, then these goes the transformation with Ecoli. But we cant know transformation yield...

11 December 2017 4,333 1 View

Gene transformation// But we will treat with calcium chloride and ice, why we choose specific vector?

If we want cell to accept vector dna in transformation, we treated with calcium chloride or chilled on ice etc.. But i have a trouble with one issue about that. Books say that ; We must choose the...

11 December 2017 9,613 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View