i wonder that logic of bioinformatic tools of ''protein to dna''.
if i want to make hybridization probe for interested amino acid sequence, what are the steps that i have to do ?
Hi, i have made hydrolysis of peptide from small protein to learn Computational Chemistry basics. While i try to find Transition state of hydrolysis, i saw double imaginary frequency but one of...
31 December 2018 923 3 View
I couldn't find a sufficiently detailed resource. This may be a very simple question for the social environment. These cells when I isolate cells from a particular tissue; Could it be from...
09 October 2018 3,646 4 View
When we use salts for precipitation of proteins for seperation process. Salting out were made by salts but if we block interactions of water and proteins; protein's structure have to be destroyed...
02 March 2018 9,237 3 View
Can be possible the transcription factor's dna binding domain's binding to two or more different DNA sequence in different(or also same) enhancers? Or there is no exception, it must be specific...
01 February 2018 2,804 3 View
Books say 3 possibilities for this situation as insert within Lacz, insert outside Lacz, no inserted vectors, then these goes the transformation with Ecoli. But we cant know transformation yield...
11 December 2017 4,404 1 View
When the aminoacid environment were changed with adding OH or etc., Aminoacid N terminal were affected by H amount and in determined pKa, they loose hydrogen from N terminal. NH3 goes Nh2. My...
11 December 2017 8,152 3 View
If we want cell to accept vector dna in transformation, we treated with calcium chloride or chilled on ice etc.. But i have a trouble with one issue about that. Books say that ; We must choose the...
11 December 2017 9,703 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View