Aprotinin is a protease inhibitor and I have my sample in very dilute form. I can concentrate and use but need to know what is the least amount of aprotinin this assay can predict.
BAEE is used as substrate in titrimetric assay.
Adam B Shapiro
The answer would depend on the conditions of the assay (buffer composition, pH, salt concentration, temperature, the signal-to-noise ratio in the assay, and most importantly, the protease being inhibited. If, for example, the concentration of aprotinin needed to produce 50% inhibition in the assay is 20 nM and your assay allows you to detect 50% inhibition convincingly, then 20 nM aprotinin would be detectable.
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