Developing hCG, FSH, LH biosimilar ?

Hi, We into developing hCG, FSH, LH biosimilar expressing CHO clones. Want to collaborate, or seek consultancy from the experts in the field. regards,

08 September 2018 4,072 0 View

Immortal heterohybridoma line secreting monoclonal anti-D antibodies ?

If any scientist ihas developed a Immortal heterohybridoma line secreting monoclonal anti-D antibodies, we are keen to collaborate to take the research to clinical level for conducting the...

08 September 2018 4,628 0 View

TGE is working but not SGE in DG44 cells ?

Our lab is working on developing the stable gene expressing clone in DG44 cells,. After transfection, transient gene expression is working, after growing the cells in the media with out GHT and...

07 August 2018 7,212 3 View

Looking for retroviral vector system for transducing DG44 cells ?

Hi We are Looking for retroviral vector system for transducing DG44 cells with GOI to produce expressing clone. Suggestion, collaboration are welcome.

02 March 2018 6,452 1 View

Want to learn the 5'-ITR and 3'-ITR sequence of the Sleeping beauty transposon ?

Want to learn/know the 5'-ITR and 3'-ITR sequence of the Sleeping beauty transposon for designing the experiments working with Sleeping beauty transposase.

10 November 2017 3,667 3 View

Expression improvement in0 DG44 CHO cells and NANA glycosylation ?

Working on the DG44 CHO cells for recombinant protein expression, we have been getting the low expression of 0.5ug/ml recombinant protein and also Gal-a(1,3)Gal glycosylation of protein....

08 September 2016 8,016 1 View

Why restriction enzyme Sap1 does not cut the circular plasmid ?

06 July 2016 8,177 3 View

Want to express hCG (alfa hCG + beta hCG) using human albumin signal peptide ?

Beta hCG expression is not seen, took of native signal peptide (20aa) of beta-hCG and replaced with human albumin signal peptide (18aa). Still B-hCG expression is not seen in the HEK293 cells. We...

03 April 2016 6,363 2 View

Two hundred USD Award for designing the Plasmid ?

We are working with pDUO2 plasmid and after transfecting it getting low expression of protein. I want to place A2UCOE sequence [HNRPA2B1 and CBX3] before the promoter for better expression, if any...

06 July 2015 3,510 2 View

How do I introduce UCOE region before the hCMV promoter in the plasmid?

Want to introduce UCOE region before the hCMV promoter in the pDUO2 plasmid already cloned with gene alpha and beta at MCS1 and MCS2,how to do this.

06 July 2015 5,717 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View