What is the difference in running a E.Coli Fermentor and Mammalian CHO cell bioreactor ?

12 December 2017 0 5K Report

what is the difference in running a E.Coli Fermentor and Mammalian CHO cell bioreactor ?

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Developing hCG, FSH, LH biosimilar ?

Hi, We into developing hCG, FSH, LH biosimilar expressing CHO clones. Want to collaborate, or seek consultancy from the experts in the field. regards,

08 September 2018 4,165 0 View

Immortal heterohybridoma line secreting monoclonal anti-D antibodies ?

If any scientist ihas developed a Immortal heterohybridoma line secreting monoclonal anti-D antibodies, we are keen to collaborate to take the research to clinical level for conducting the...

08 September 2018 4,702 0 View

TGE is working but not SGE in DG44 cells ?

Our lab is working on developing the stable gene expressing clone in DG44 cells,. After transfection, transient gene expression is working, after growing the cells in the media with out GHT and...

07 August 2018 7,281 3 View

Looking for retroviral vector system for transducing DG44 cells ?

Hi We are Looking for retroviral vector system for transducing DG44 cells with GOI to produce expressing clone. Suggestion, collaboration are welcome.

02 March 2018 6,526 1 View

Want to learn the 5'-ITR and 3'-ITR sequence of the Sleeping beauty transposon ?

Want to learn/know the 5'-ITR and 3'-ITR sequence of the Sleeping beauty transposon for designing the experiments working with Sleeping beauty transposase.

10 November 2017 3,743 3 View

Expression improvement in0 DG44 CHO cells and NANA glycosylation ?

Working on the DG44 CHO cells for recombinant protein expression, we have been getting the low expression of 0.5ug/ml recombinant protein and also Gal-a(1,3)Gal glycosylation of protein....

08 September 2016 8,082 1 View

Why restriction enzyme Sap1 does not cut the circular plasmid ?

Am working with cloning, and plasmid has an Sap1 restriction site at Forward and reverse (purchased the cut plasmid from an company, they call it Electracloning) and the insert gene has forward...

06 July 2016 8,268 3 View

Want to express hCG (alfa hCG + beta hCG) using human albumin signal peptide ?

Beta hCG expression is not seen, took of native signal peptide (20aa) of beta-hCG and replaced with human albumin signal peptide (18aa). Still B-hCG expression is not seen in the HEK293 cells. We...

03 April 2016 6,444 2 View

How do I introduce UCOE region before the hCMV promoter in the plasmid?

Want to introduce UCOE region before the hCMV promoter in the pDUO2 plasmid already cloned with gene alpha and beta at MCS1 and MCS2,how to do this.

06 July 2015 5,780 2 View

Two hundred USD Award for designing the Plasmid ?

We are working with pDUO2 plasmid and after transfecting it getting low expression of protein. I want to place A2UCOE sequence [HNRPA2B1 and CBX3] before the promoter for better expression, if any...

06 July 2015 3,584 2 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Why running a restart analysis in Abaqus in Ubuntu OS gives an error as attached when running the same job in Windows doesn't give any error and runs?

I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...

29 July 2024 9,572 3 View

How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

28 July 2024 6,553 1 View

How do I run a dead well test for a QuantStudio 5 qPCR machine?

I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...

24 July 2024 8,172 1 View

VECM Model: Positive/insignificant CointEq1 ?

Hello everyone, I am conducting

22 July 2024 8,085 11 View

Merge Intensity Data from 2 runs?

Hi, We have collected several images for two independent runs of an experiment using two different confocal microscopes and settings. Thus, the intensity values collected through ImageJ were...

22 July 2024 8,722 0 View

What is the solvent system required to run tlc for calixarenes?

If you don't understand my question let me know

21 July 2024 563 1 View